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cd73 surface marker  (Thermo Fisher)


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    Structured Review

    Thermo Fisher cd73 surface marker
    Cd73 Surface Marker, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd73+surface+marker/anti+cd73/pmc11120611-67-22-25
    Average 90 stars, based on 1 article reviews
    cd73 surface marker - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Flow Cytometry:

    Article Title: Isolation, Characterization, and Transplantation of Bone Marrow-Derived Cell Components with Hematopoietic Stem Cell Niche Properties
    Article Snippet: In addition, surface marker analysis on day 10 showed that they were positive for CD24, CD29, CD44, and CD34 and negative for CD31, CD105, CD73, and CD90; CD45 (all from eBioscience) was weakly expressed ( ).

    Article Title: Therapeutic Effect of Rapamycin-Loaded Small Extracellular Vesicles Derived from Mesenchymal Stem Cells on Experimental Autoimmune Uveitis
    Article Snippet: According to the criteria proposed by ISCT , flow cytometry was used to identify the surface markers of MSCs, including CD73 (eBioscience, USA), CD90 (eBioscience), CD45 (eBioscience), and CD34 (eBioscience).

    Article Title: Mechanisms of human umbilical cord mesenchymal stem cells-derived exosomal lncRNA GAS5 in alleviating EMT of HPMCs via Wnt/β-catenin signaling pathway
    Article Snippet: Flow analysis revealed MSC-specific surface markers, CD73, CD90, and CD14 (eBioscience, USA).

    Article Title: miR-103-3p regulates the differentiation of bone marrow mesenchymal stem cells in myelodysplastic syndrome
    Article Snippet: The MSCs were positive for CD73, CD90, and CD105 and negative for CD34, CD45, and CD14 (eBiosciences, USA) (Mushahary et al., 2018), and suitable isotypic controls were used to characterize the MSCs.

    Article Title: Evaluation of Human Platelet Lysate as an Alternative to Fetal Bovine Serum for Potential Clinical Applications of Stem Cells from Human Exfoliated Deciduous Teeth
    Article Snippet: To ascertain the surface markers of hPL-SHEDs and FBS-SHEDs, flow cytometry analysis was conducted employing specific markers including CD14, CD29, CD44, and CD73 (obtained from Thermofisher, catalog numbers 11-0149-42, 11-0299-42, 11-0441-82, and 11-0739-42, respectively).

    Cell Culture:

    Article Title: Isolation, Characterization, and Transplantation of Bone Marrow-Derived Cell Components with Hematopoietic Stem Cell Niche Properties
    Article Snippet: In addition, surface marker analysis on day 10 showed that they were positive for CD24, CD29, CD44, and CD34 and negative for CD31, CD105, CD73, and CD90; CD45 (all from eBioscience) was weakly expressed ( ).

    Article Title: Therapeutic Effect of Rapamycin-Loaded Small Extracellular Vesicles Derived from Mesenchymal Stem Cells on Experimental Autoimmune Uveitis
    Article Snippet: According to the criteria proposed by ISCT , flow cytometry was used to identify the surface markers of MSCs, including CD73 (eBioscience, USA), CD90 (eBioscience), CD45 (eBioscience), and CD34 (eBioscience).

    Article Title: Mechanisms of human umbilical cord mesenchymal stem cells-derived exosomal lncRNA GAS5 in alleviating EMT of HPMCs via Wnt/β-catenin signaling pathway
    Article Snippet: Flow analysis revealed MSC-specific surface markers, CD73, CD90, and CD14 (eBioscience, USA).

    Article Title: miR-103-3p regulates the differentiation of bone marrow mesenchymal stem cells in myelodysplastic syndrome
    Article Snippet: The MSCs were positive for CD73, CD90, and CD105 and negative for CD34, CD45, and CD14 (eBiosciences, USA) (Mushahary et al., 2018), and suitable isotypic controls were used to characterize the MSCs.

    Article Title: Evaluation of Human Platelet Lysate as an Alternative to Fetal Bovine Serum for Potential Clinical Applications of Stem Cells from Human Exfoliated Deciduous Teeth
    Article Snippet: To ascertain the surface markers of hPL-SHEDs and FBS-SHEDs, flow cytometry analysis was conducted employing specific markers including CD14, CD29, CD44, and CD73 (obtained from Thermofisher, catalog numbers 11-0149-42, 11-0299-42, 11-0441-82, and 11-0739-42, respectively).

    Staining:

    Article Title: Isolation, Characterization, and Transplantation of Bone Marrow-Derived Cell Components with Hematopoietic Stem Cell Niche Properties
    Article Snippet: In addition, surface marker analysis on day 10 showed that they were positive for CD24, CD29, CD44, and CD34 and negative for CD31, CD105, CD73, and CD90; CD45 (all from eBioscience) was weakly expressed ( ).

    Article Title: Therapeutic Effect of Rapamycin-Loaded Small Extracellular Vesicles Derived from Mesenchymal Stem Cells on Experimental Autoimmune Uveitis
    Article Snippet: According to the criteria proposed by ISCT , flow cytometry was used to identify the surface markers of MSCs, including CD73 (eBioscience, USA), CD90 (eBioscience), CD45 (eBioscience), and CD34 (eBioscience).

    Article Title: Mechanisms of human umbilical cord mesenchymal stem cells-derived exosomal lncRNA GAS5 in alleviating EMT of HPMCs via Wnt/β-catenin signaling pathway
    Article Snippet: Flow analysis revealed MSC-specific surface markers, CD73, CD90, and CD14 (eBioscience, USA).

    Article Title: miR-103-3p regulates the differentiation of bone marrow mesenchymal stem cells in myelodysplastic syndrome
    Article Snippet: The MSCs were positive for CD73, CD90, and CD105 and negative for CD34, CD45, and CD14 (eBiosciences, USA) (Mushahary et al., 2018), and suitable isotypic controls were used to characterize the MSCs.

    Article Title: Evaluation of Human Platelet Lysate as an Alternative to Fetal Bovine Serum for Potential Clinical Applications of Stem Cells from Human Exfoliated Deciduous Teeth
    Article Snippet: To ascertain the surface markers of hPL-SHEDs and FBS-SHEDs, flow cytometry analysis was conducted employing specific markers including CD14, CD29, CD44, and CD73 (obtained from Thermofisher, catalog numbers 11-0149-42, 11-0299-42, 11-0441-82, and 11-0739-42, respectively).



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    Fig. 5 Transcriptome analysis of the role of TET3 in HUCMSC differentiation towards oligodendrocyte progenitor cells (OPCs). a Heatmap showing upregulation of genes promoting oligodendrocyte lineage formation and differentiation and downregulation of inhibitory genes. b Correlation analysis between TET3 expression and multiple genes related to the oligodendrocyte lineage. c–g Positive correlation between TET3 and (c) APC, (d) SOX5, (e) ASH1L, (f) GALC, and (g) IGF1. h–j Negative correlation between TET3 and (h) IL1B, (i) ENG (CD105), and (j) NT5E <t>(CD73).</t> k Suppression of pathways inhibiting oligodendrocyte lineage formation during differentiation. l Negative correlation between TET3 and the Wnt signaling pathway
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    Fig. 5 Transcriptome analysis of the role of TET3 in HUCMSC differentiation towards oligodendrocyte progenitor cells (OPCs). a Heatmap showing upregulation of genes promoting oligodendrocyte lineage formation and differentiation and downregulation of inhibitory genes. b Correlation analysis between TET3 expression and multiple genes related to the oligodendrocyte lineage. c–g Positive correlation between TET3 and (c) APC, (d) SOX5, (e) ASH1L, (f) GALC, and (g) IGF1. h–j Negative correlation between TET3 and (h) IL1B, (i) ENG (CD105), and (j) NT5E <t>(CD73).</t> k Suppression of pathways inhibiting oligodendrocyte lineage formation during differentiation. l Negative correlation between TET3 and the Wnt signaling pathway
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    Fig. 5 Transcriptome analysis of the role of TET3 in HUCMSC differentiation towards oligodendrocyte progenitor cells (OPCs). a Heatmap showing upregulation of genes promoting oligodendrocyte lineage formation and differentiation and downregulation of inhibitory genes. b Correlation analysis between TET3 expression and multiple genes related to the oligodendrocyte lineage. c–g Positive correlation between TET3 and (c) APC, (d) SOX5, (e) ASH1L, (f) GALC, and (g) IGF1. h–j Negative correlation between TET3 and (h) IL1B, (i) ENG (CD105), and (j) NT5E <t>(CD73).</t> k Suppression of pathways inhibiting oligodendrocyte lineage formation during differentiation. l Negative correlation between TET3 and the Wnt signaling pathway
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    Representative histograms for flow cytometry analysis of the expression of mesenchymal stem cell (MSC) markers in cultured periodontal ligament stem cells. After five passages cells were positive for common MSC markers such as CD105 and CD90 and negative for the leukocyte antigen CD45 in both serum-free (a) and serum-containing (b) media. While <t>CD73</t> showed an adequate expression in the absence of fetal bovine serum. The red lines represent isotype control Immunoglobulin G expression and green lines depict the marker expression.
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    Image Search Results


    Fig. 5 Transcriptome analysis of the role of TET3 in HUCMSC differentiation towards oligodendrocyte progenitor cells (OPCs). a Heatmap showing upregulation of genes promoting oligodendrocyte lineage formation and differentiation and downregulation of inhibitory genes. b Correlation analysis between TET3 expression and multiple genes related to the oligodendrocyte lineage. c–g Positive correlation between TET3 and (c) APC, (d) SOX5, (e) ASH1L, (f) GALC, and (g) IGF1. h–j Negative correlation between TET3 and (h) IL1B, (i) ENG (CD105), and (j) NT5E (CD73). k Suppression of pathways inhibiting oligodendrocyte lineage formation during differentiation. l Negative correlation between TET3 and the Wnt signaling pathway

    Journal: Journal of translational medicine

    Article Title: TET3-facilitated differentiation of human umbilical cord mesenchymal stem cells into oligodendrocyte precursor cells for spinal cord injury recovery.

    doi: 10.1186/s12967-024-05929-7

    Figure Lengend Snippet: Fig. 5 Transcriptome analysis of the role of TET3 in HUCMSC differentiation towards oligodendrocyte progenitor cells (OPCs). a Heatmap showing upregulation of genes promoting oligodendrocyte lineage formation and differentiation and downregulation of inhibitory genes. b Correlation analysis between TET3 expression and multiple genes related to the oligodendrocyte lineage. c–g Positive correlation between TET3 and (c) APC, (d) SOX5, (e) ASH1L, (f) GALC, and (g) IGF1. h–j Negative correlation between TET3 and (h) IL1B, (i) ENG (CD105), and (j) NT5E (CD73). k Suppression of pathways inhibiting oligodendrocyte lineage formation during differentiation. l Negative correlation between TET3 and the Wnt signaling pathway

    Article Snippet: Expression of surface markers CD73 (13160, Cell Signaling Technology), CD105 (4335, Cell Signaling Technology), immunoglobulin (Ig)G1 (5415, Cell Signaling Technology), IgG2a (61656, Cell Signaling Technology), CD45 (13917, Cell Signaling Technology), and HLA-DR (78397, Cell Signaling Technology) were identified by flow Cytometer (FACSA Canto II; BD Biosciences, USA).

    Techniques: Expressing

    Representative histograms for flow cytometry analysis of the expression of mesenchymal stem cell (MSC) markers in cultured periodontal ligament stem cells. After five passages cells were positive for common MSC markers such as CD105 and CD90 and negative for the leukocyte antigen CD45 in both serum-free (a) and serum-containing (b) media. While CD73 showed an adequate expression in the absence of fetal bovine serum. The red lines represent isotype control Immunoglobulin G expression and green lines depict the marker expression.

    Journal: Dental Research Journal

    Article Title: Isolation and characterization of human periodontal ligament stem cells under the terms of use in clinical application: A pilot study

    doi:

    Figure Lengend Snippet: Representative histograms for flow cytometry analysis of the expression of mesenchymal stem cell (MSC) markers in cultured periodontal ligament stem cells. After five passages cells were positive for common MSC markers such as CD105 and CD90 and negative for the leukocyte antigen CD45 in both serum-free (a) and serum-containing (b) media. While CD73 showed an adequate expression in the absence of fetal bovine serum. The red lines represent isotype control Immunoglobulin G expression and green lines depict the marker expression.

    Article Snippet: To verify the identity of the resulting cells, expression of the surface markers CD90, CD105, and CD73 (all from Millipore), which are known as minimal criteria of MSCs and CD45 as an indicating marker of hematopoietic cells were analyzed by flow cytometry.

    Techniques: Flow Cytometry, Expressing, Cell Culture, Control, Marker

    Gene expression in cultured periodontal ligament stem cells. Reverse transcription-polymerase chain reaction was applied to compare the expression levels of CD73, CD105, CD90, and CD45 between two culture conditions. Data revealed a normal expression of mesenchymal stem cell-specific genes by cultured periodontal ligament stem cells in serum-free medium. Although the expression of CD73 was week in + fetal bovine serum culture condition.

    Journal: Dental Research Journal

    Article Title: Isolation and characterization of human periodontal ligament stem cells under the terms of use in clinical application: A pilot study

    doi:

    Figure Lengend Snippet: Gene expression in cultured periodontal ligament stem cells. Reverse transcription-polymerase chain reaction was applied to compare the expression levels of CD73, CD105, CD90, and CD45 between two culture conditions. Data revealed a normal expression of mesenchymal stem cell-specific genes by cultured periodontal ligament stem cells in serum-free medium. Although the expression of CD73 was week in + fetal bovine serum culture condition.

    Article Snippet: To verify the identity of the resulting cells, expression of the surface markers CD90, CD105, and CD73 (all from Millipore), which are known as minimal criteria of MSCs and CD45 as an indicating marker of hematopoietic cells were analyzed by flow cytometry.

    Techniques: Gene Expression, Cell Culture, Reverse Transcription, Polymerase Chain Reaction, Expressing

    Polymerase chain reaction primer sequences

    Journal: Dental Research Journal

    Article Title: Isolation and characterization of human periodontal ligament stem cells under the terms of use in clinical application: A pilot study

    doi:

    Figure Lengend Snippet: Polymerase chain reaction primer sequences

    Article Snippet: To verify the identity of the resulting cells, expression of the surface markers CD90, CD105, and CD73 (all from Millipore), which are known as minimal criteria of MSCs and CD45 as an indicating marker of hematopoietic cells were analyzed by flow cytometry.

    Techniques: Polymerase Chain Reaction, Sequencing